Colin Northward: Rope suction cup attaches to wall.
Nekka the Eagle: The vine-like Rope makes an organic attaching noise.
LISE Project: The electrical plug at the end of the Rope makes a soft attaching noise as it inserts into the background.
Manfred Tunnel: Sound of Rope "Digging" into wall.
Tina Flan: The carabiner clip at the end of the Rope "clanks" onto the background with a loud metal sound.
Valerie Crump: The chewed gum at the end of the Rope sticks to the wall.
Au: Deploying plays a loud, tube like unfurling noise.
Pilot: The arm-like Rope makes a rubbery flailing sound as it is thrown upward.
Classic Guy: The Rope makes the same sound effects as it did in Spelunky Classic.
Some characters have their own custom Whips. These whips function identically to the default whip, and only appear different. Notably, Hired Hands and the Eggplant Child also have their very own custom whip.
When a Spelunker dies in co-op, they will drop a Corpse Bag and become a Ghist. The colors and features of the Ghist resemble the player character that died.
Player Ghists can use the attack button to hold their breath and release a puff of air, knocking anything forward. If a player Ghist holds their breath for over 2.5 seconds, they will be able to shoot a short-ranged ice blast that acts identical to that of the Freeze Ray's.
Pressing the "bomb" button while playing as a ghist will boost them forward.
Pressing the "rope" button while playing as a ghist will cause them to shake and make a loud rattling noise. This has no effect on gameplay.
Pressing the "jump" button will cause the player's ghist to spin. This also has no effect on gameplay.
Much of the Spelunky 2 cast is either related to or made up of former Spelunkers. For example, it is implied that Little Jay and Valerie Crump are The Round Boy and The Round Girl from the original adventure or at the very least related to them, with both having lost weight and gaining more individuality.
Colin Northward is based on Colin Northway, a friend of Spelunky and Spelunky 2's lead designer Derek Yu.
Roffy D. Sloth's namesake may be a reference to One Piece's main character, 'Monkey D. Luffy'
Van Horsing, who was previously a Spelunker in Spelunky HD, returns as an NPC in Spelunky 2. His namesake and appearance is also a reference to Van Helsing, a fictional character from the 1897 gothic horror novel Dracula. Both serve as vampire hunters in their respective appearances.
Tina Flan's description is a reference to how she was the character used by the Youtuber/Streamer BananasaurusRex during the first successfully recorded solo eggplant run.
This reference is taken further by a unique dialogue during her interaction with the Eggplant King, as he says "Ah, you've come to finish the job!" if he is killed by Tina.
The Pilot appears to be a reference to one of the games in the upcoming UFO 50 (also by Mossmouth), known as Campanella. He appears here in the game's trailer.
Demi Von Diamonds is a reference to the Damsel from Spelunky Classic and HD. Her dialogue hints that she is the same character from the first game, seeming to know Guy and the original Yang from the first game.
Dirk Yamaoka and Sparrow are siblings. If Dirk Yamaoka meets Sparrow, she will say "Big brother! I never thought I'd catch you thieving..."
Dirk Yamaoka's last name is the same as Chris Yamaoka, a person given Special Thanks to in the Credits.
Au is the Periodic Symbol for Gold, hence the Golden Monk's name.
Although "Cyan" does not make a reappearance in Spelunky 2, he is referenced in the credits, as Andy Hull's cartoon icon has him wearing the same outfit as him.